This revolutionary methodology, which we and the field have been trying to develop for the past 20 years, is going to change the way we define gene specific regulation of transcription. Now we can identify a region of the genome that is a diseased region and molecularly define what's really going on in there, said Shilatifard.
Traditional methods like chromatin immunoprecipitation followed by sequencing allowed us to identify where specific proteins bind across the genome. However, these techniques are limited because they typically analyze one protein at a time, require large numbers of cells and capture only static snapshots of protein-DNA interactions, said Bercin Cenik.
The new technique allows scientists to map a complete set of proteins interacting with a specific genomic region in mammalian cells with higher specificity, sensitivity and temporal control than previous methods.
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