The careful selection of sample sizes, ranging from n=2 to n=200 across various organisms, reflects the rigor in evaluating metabolite levels and physiological metrics. Ensuring sample comparability, all experiments adhered to consistent housing conditions for animal subjects and controlled environments for cell cultures.
No statistical methods pre-determined the sample sizes, emphasizing a practical approach that focused on replicability. Each experimental design included appropriate controls and utilized randomization to reduce bias, illustrating a commitment to accurate and reliable data collection.
Collection
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