The chosen sample sizes followed standard practices in this research area, utilizing n = 4-5 for metabolic evaluations and extending to 200 samples for lifespan analysis, ensuring reliable data.
Randomization was systematically applied in both animal and cell experiments, enhancing the validity of results, such as when sex- and age-matched animals received treatments in a balanced manner.
Experimental designs included robust controls and parallel processing of samples to minimize bias, with successful replication of experiments contributing to the overall credibility of the findings.
No statistical methods were used to predetermine sample sizes, yet comprehensive replication affirmed the experimental design's reliability and consistency across various biological models.
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