Regenerating secrets for Azure DevOps OAuth applications - Azure DevOps Blog
Briefly

A valid, active client secret is critical for getting a refresh token to continue using your app. Once the secret has expired, you will also no longer be able to get access and refresh tokens needed to access Azure Devops APIs through this app.
When you generate a new client secret, the previous client secret will expire and so will all previously minted access tokens for that app. Please bear that in mind when you schedule any necessary outage time while your new secret replaces your old one.
Read at Azure DevOps Blog
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