The study utilized multiple strains of C57BL/6 mice, including genetically engineered models like OT-I and Cd8CreIfngfl/fl, to explore immune response mechanisms and tumor interactions. Mice were housed in controlled conditions at St. Jude Children's Research Hospital, and all experimental procedures followed strict ethical regulations and institutional approval. The generation of bone marrow chimeras allowed for the observation of immune responses, particularly regarding tumor inoculation in specific genetic backgrounds, contributing valuable insights into cancer biology and immunology.
C57BL/6 and several genetically engineered mice were used in rigorous studies under specific-pathogen-free conditions at St. Jude's, complying with ethical and institutional guidelines.
OT-I mice were crossed with Rosa26-Cas9 mice to create OT-I-Cas9 mice, allowing for targeted gene editing in research into immune responses.
Bone marrow chimeras were generated from Cd8CreIfngfl/fl or control mice, facilitating the study of tumor inoculation effects post-irradiation in Rag1−/− recipients.
The animal experimentation adhered to ethical standards and Institutional Animal Care and Use Committee regulations, ensuring humane treatment and scientifically valid methodologies.
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