This article presents the structural analysis of a unique long non-coding RNA, ROOL, which forms octameric nanocages. Using cryo-electron microscopy, the research highlights the nanocage's dimensions and stabilizing interactions, including proposed 'A-minor staples'. The study uncovers that the assembly involves intricate mechanisms like strand-swapping and kissing loops, suggesting insights into lncRNA functionality. Furthermore, the ROOL RNA can be fused with other RNA types while maintaining the nanocage structure, opening avenues for innovative RNA-based delivery systems in research and therapeutics.
The study elucidates the structure of a natural RNA nanocage formed by the novel lncRNA ROOL, revealing its potential in biotechnology applications.
Using cryo-EM, we demonstrate that ROOL forms an octameric nanocage with unique structural features, suggesting its importance in biological functions and as a prototype for RNA engineering.
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