During their two-hour spacewalk, Isaacman and Gillis will face dangerous radiation and life-threatening pressure changes as they test SpaceX's new spacesuits.
SpaceX has confirmed that the four-hour window for the spacewalk will open at 04:55am ET (09:55am BST). "All systems are looking good for the Polaris Dawn crew to perform the first spacewalk from Dragon today," it tweeted.
The Polaris Dawn mission launched from the Kennedy Space Centre in Florida on September 10 at 5:23 am ET after days of delay due to weather and a helium leak.
Yesterday, the SpaceX Dragon Crew spacecraft carried its crew to an altitude of 870 miles (1,400 km) above Earth - the highest any human has been since NASA's Apollo missions.
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