Compartment-specific ribosomal subunit processing was observed in human nucleoli with small subunit (SSU) processomes localized in fibrillar center/dense fibrillar component regions. Large subunit (LSU) pre-rRNAs transitioned to PDFC and granular component areas. Slow proliferating cells showed impaired SSU processing associated with structural changes in the nucleolus and decreased SSU outflux. Disruption of 5' external transcribed space processing altered nucleolar structures, indicating a functional interdependence. Anamniote nucleoli results differed, showcasing varied pre-rRNA processing rates and structures compared to multi-layered amniote nucleoli.
Spatiotemporal analyses indicate compartment-specific ribosomal subunit processing in human nucleoli, showing that SSU processomes are retained in fibrillar center/dense fibrillar component regions.
Slow proliferating cells displayed 5' external transcribed space-centered SSU processing impairments, with changes in nucleolar structure and SSU outflux rates.
Interventions illustrating perturbation of 5' ETS processing directly influenced structural remodeling in fibrillar centers, supporting a relationship between SSU processing and nucleolar architecture.
Anamniote nucleoli, with merged compartments, showed distinct processing characteristics, indicating differences in pre-rRNA flux compared to amniote multi-layered nucleoli.
#nucleolar-architecture #ribosome-biogenesis #rna-metabolism #cell-proliferation #spatiotemporal-analysis
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