All the pieces are there to start with to do single-molecule proteomics and identify proteins and their modifications using nanopores... There are many different ways you can identify proteins which doesn't really require the exact identification of all 20 amino acids.
In nanopore DNA sequencing, single-stranded DNA is driven through a protein pore by an electrical current. As a DNA residue traverses the pore, it disrupts the current to produce a characteristic signal that can be decoded into a sequence of DNA bases.
Proteins, however, are harder to crack... researchers are making progress.
Maglia and his team engineered a nanopore with charges positioned so that the pore could create an electro-osmotic flow that was strong enough to unfold a full-length protein and carry it through the pore.
#nanopore-sequencing #protein-analysis #post-translational-modifications #electro-osmotic-flow #biotechnology
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