The article introduces CRISPR-mediated transcriptome organization (CRISPR-TO), a novel system that allows targeted and reversible manipulation of RNA localization within living cells. By leveraging dCas13 technology, researchers can direct endogenous RNA to specific compartments, significantly advancing the study of spatial transcriptomics. Experiments in primary cortical neurons demonstrate that localized mRNAs are translated and influence neurite dynamics, paving the way for further exploration of RNA's role in cellular processes. By providing a means to disrupt the spatial transcriptome, CRISPR-TO offers a powerful tool for high-throughput functional analyses of RNA in various contexts.
CRISPR-TO is a groundbreaking tool that enables targeted localization and real-time manipulation of endogenous RNA within living cells, revealing functional implications previously unexplored.
Using CRISPR-TO, we can now control RNA localization across various subcellular compartments, enhancing understanding of dynamic processes such as translation and neurite growth.
The ability to induce and reverse RNA transport along microtubules paves the way for novel investigations into RNA behavior and its roles in cellular processes.
Our findings suggest that specific mRNA localization is critical for neurite outgrowth, illustrating the importance of spatial transcriptomics in understanding neuronal behavior.
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