""We had believed that we could create an infinite number of clones. That is why these results are so disappointing," study senior author Teruhiko Wakayama stated, highlighting the unexpected limitations encountered in the cloning process."
""At this point, we have no ideas for overcoming this limitation. I believe we need to develop a new method that fundamentally improves nuclear transfer technology," Wakayama added, indicating the need for advancements in cloning techniques."
""Starting with the 27th generation, they became less fertile as they gave birth to smaller litters, and they also had larger placentas. All the while, more and more of them kept dying," illustrating the gradual decline in clone viability."
""By the 58th generation, all of the recloned mice died the day after being born, their precise cause of death unknown," underscoring the severe consequences of prolonged cloning efforts."
A two-decade experiment in Japan involved cloning a female mouse for 58 generations. Initially, clones appeared healthy, but by the 27th generation, fertility declined and mortality increased. By the 58th generation, all clones died immediately after birth, despite no visible abnormalities. This study indicates a hard limit to mammalian cloning, disappointing researchers who hoped for infinite cloning possibilities. Senior author Teruhiko Wakayama emphasized the need for new methods to improve cloning technology.
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