Antibiotic persistence allows rare bacterial cells to survive high antibiotic concentrations, often by entering a dormant state, complicating treatment and eradication.
Recent advances in single-cell RNA sequencing enabled the identification of Klebsiella pneumoniae persisters. Understanding these states in naive populations is crucial for broader insight.
Our research involved creating a single-cell atlas of E. coli growth transitions, revealing a unique persister cluster characterized by translational deficiency distinct from other phases.
Through CRISPR-interference screening, we pinpointed several gene products, such as Lon protease and YqgE, contributing to the formation of antibiotic persisters.
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