Peterborough United will face Northampton Town at home in the last 32 of the Vertu Trophy after progressing through the group stage with a perfect record.
The tournament has seen familiar clubs face off, including Wycombe, who play Swindon Town, while Aston Villa U21s will challenge Blackpool in an away match.
Notably, Birmingham City returns to the competition after a long absence, demonstrating a shift as they gear up for their match against Exeter City in Devon.
Withdrawals of Premier League academy teams highlight the competition's focus on League One and League Two clubs, with Bolton winning the trophy last season.
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