"This tragedy has deeply affected our team, and we are all grieving the loss of these incredible animals," the Wild Felid Advocacy Center of Washington wrote in a post on Facebook.
"We've never had anything like it; they usually die basically of old age... Not something like this, it's a pretty wicked virus," said Mark Mathews, director of the sanctuary.
"Cats are particularly vulnerable to this virus, which can cause subtle initial symptoms but progress rapidly, often resulting in death within 24 hours due to pneumonia-like conditions," states the sanctuary.
The news comes as bird flu continues to spread among cattle and poultry in the US, while also severely infecting at least one human.
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