Here, using an optimized 3D suspension culture system, we have successfully advanced the in vitro culture of a stem cell-derived monkey blastoid to day 25. Morphological and histological analyses showed that these monkey embryoids underwent gastrulation and largely recapitulated key developmental events of the late gastrulation stage observed in vivo, with the appearance of a neural plate, haematopoietic system, allantois, primitive gut, primordial germ cells, yolk sac structures and progenitors of other organs, excluding trophoblast derivatives.
Long non-coding RNAs (lncRNAs) are RNA molecules that do not encode proteins, which led to their dismissal by many scientists as unimportant as it relates to DNA processes. For years, laboratories lacked the tools to study these molecules, and their roles in cellular processes remained obscure. However, the new study demonstrates that lncRNAs are far from inert, said Jhumku Kohtz, PhD, research professor in the Ken and Ruth Davee Department of Neurology's Divison of Comprehensive Neurology and senior author of the study.