"Mammalian oocytes are filled by fibric structure called cytoplasmic lattice (CPL), essential for oocyte maturation and early embryonic development. CPL comprises subcortical maternal complex (SCMC) and multiple components, including PADI6. Despite its discovery in the 1960s, the molecular architecture and assembly mechanisms of CPL have remained poorly understood."
"CPL is composed of repeating U-shaped basket (UB) and adapter ring (AR)-featured units, forming a filamentous architecture. AR adopts a two-fold symmetric conformation, containing two NLRP4f, four SCMC and two ZBED3 subunits circularized via two distinct interaction clusters."
"The UB is anchored by PADI6, a didecamer composed of ten homodimers assembled by two back-to-back pentamers, each forming the lateral side of UB. The underfoot base and up-down sides of the UB are formed by multiple central-symmetric assemblies."
The cytoplasmic lattice (CPL) is a fibrous structure filling mammalian oocytes that plays a critical role in oocyte maturation and early embryonic development. Despite being discovered in the 1960s, its molecular architecture and assembly mechanisms remained unclear. Using cryo-electron microscopy on mouse oocytes, researchers identified 14 constitutive protein subunits and determined that CPL consists of repeating U-shaped basket (UB) and adapter ring (AR) units forming a filamentous architecture. The adapter ring contains NLRP4f, SCMC, and ZBED3 subunits, while the U-shaped basket is anchored by PADI6 didecamers composed of ten homodimers arranged in back-to-back pentamers, with additional protein assemblies forming the structural base.
#cryo-electron-microscopy #cytoplasmic-lattice-structure #oocyte-maturation #protein-architecture #reproductive-biology
Read at www.nature.com
Unable to calculate read time
Collection
[
|
...
]