"Mice were group-housed on a 12 h light:12 h dark cycle with ad libitum access to food (Purina Rodent Chow, 5001) and water unless otherwise noted. Temperature (21.5-22.3 °C) and humidity (50 ± 15%) were controlled. Group-housed adult male and female mice (at least eight weeks old) were used for experiments. Npy1r-cre (Jackson Laboratory 030544, B6.Cg-Npy1rtm1.1(cre/GFP)Rpa/J (ref. ), Npy-Flp (Jackson Laboratory 030211, B6.Cg-Npytm1.1(flpo)Hze/J), Pdyn-IRES-cre (Jackson Laboratory 027958, B6;129S- Pdyntm1.1(cre)Mjkr/LowlJ),"
"Genotyping of the Y1-lox/lox mice was performed using primers and conditions provided by Jackson Laboratory. All other strains were genotyped by Transnetyx. Sample sizes were chosen based on standards in the neuroscience field and our prior data. All mice were habituated to handling and experimental conditions prior to experiments. For within-subject behavioural analyses, all mice received all experimental conditions. Conditions were counter balanced unless otherwise noted, and order was randomly assigned. For between-subject analyses, mice were randomly assigned to experimental condition."
Mice were group-housed on a 12 h light:12 h dark cycle with ad libitum access to food and water. Temperature and humidity were controlled. Adult male and female mice (at least eight weeks old) from multiple genetically modified and wild-type strains were used. Genotyping for Y1-lox/lox mice used primers and conditions provided by Jackson Laboratory, and other strains were genotyped by Transnetyx. Sample sizes were chosen based on field standards and prior data. Mice were habituated to handling and experimental conditions. Within-subject experiments used counterbalanced, randomly ordered conditions, while between-subject experiments used random assignment and appropriate blinding.
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