This article discusses a novel approach to liver disease modeling using a multicellular organoid system that integrates adult hepatocytes, cholangiocytes, and mesenchymal cells. The newly developed assembloids replicate the complex architecture of the liver's periportal region and demonstrate functional features such as a bile canaliculi network. Importantly, adjustments in the ratio of portal mesenchymal cells can induce fibrotic-like states, providing valuable insights for understanding cholestatic injury and biliary fibrosis. These findings could significantly enhance the study of liver diseases and therapeutic strategies.
Operating on a multicellular organoid system of hepatocytes, cholangiocytes, and mesenchymal cells, we successfully modeled cholestatic injury and biliary fibrosis.
The engineered assembloids demonstrate a functioning bile canaliculi network that closely reflects in vivo liver architecture and cellular interactions.
Our research indicates that manipulating the composition of mesenchymal cells in liver models can initiate a fibrotic-like state without the influence of the immune system.
This innovative organoid system provides a robust platform for investigating liver disease mechanisms and potential therapeutic strategies in a controlled environment.
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