"The breadth of ubiquitinated non-protein substrates, their abundance, and cellular roles are currently unclear, since current ubiquitinomic and proteomic techniques are blind to non-proteinaceous modifications."
"We report Non-Protein Ub-clipping (NoPro-clipping) as a mass-spectrometry-based technique that combines ubiquitin clippases with sortase labelling, unveiling a vast new canvas of ubiquitin modifications."
"Our findings reveal ubiquitinated glycogen in any glycogen-containing tissue in mice, with the highest abundance found in liver and skeletal muscle."
Non-Protein Ub-clipping (NoPro-clipping) is a novel mass-spectrometry-based technique that identifies ubiquitin modifications on non-protein substrates. Current methods overlook these modifications, limiting understanding of their roles. This technique combines ubiquitin clippases with sortase labeling, allowing for both targeted and untargeted workflows. Findings indicate that ubiquitinated glycogen is present in various tissues, particularly in the liver and skeletal muscle, suggesting a broader significance of ubiquitination beyond traditional protein substrates.
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