"B16-F0 (ATCC; CRL-6322) and its LN metastatic derivatives: NBF0-LN1-18IL, NBF0-LN7-1112AR, NBF0-LN7-1120BL, NBF0-LN7-1134BL, NBF0-LN8-1194BR, NBF0-LN8-1198AR, NBF0-LN8-1205BL, NBF0-LN9-1315BL and NBF0-LN9-1358IR-were provided by the Reticker-Flynn Laboratory. For simplicity, these cell lines are referred to throughout the manuscript as: B16-F0, LN1-18IL, LN7-1112AR, LN7-1120BL, LN7-1134BL, LN8-1194BR, LN8-1198AR, LN8-1205BL, LN9-1315BL and LN9-1358IR, respectively. B16F10 wild-type (WT), B16F10 Fsp1-KO and B16F10 Gpx4-KO cells were obtained from the Conrad Laboratory."
"B16-F0 Fsp1-KO, LN7-1134BL Fsp1-KO, LN9-1315BL Fsp1-KO, B16-F0 Gclc-overexpression, LN7-1134BL Gclc-overexpression, B16-F0 Gclc-KO and B16-F0 Nrf2- overexpression lines were generated in this study. Human melanoma cell lines MeWo, SK-MEL-5, A375, murine melanoma lines Yale University Melanoma Model (YUMM) 3.3 and YUMM 5.2, and HEK293T cells were purchased from ATCC. All cell lines were cultured in Dulbecco's modified Eagle's medium (DMEM; Thermo Fisher Scientific, 11885076) supplemented with 10% FBS (Thermo Fisher Scientific, 26400044) and 1% penicillin-streptomycin (Thermo Fisher Scientific, 15140122)."
"Chemicals RSL3 (HY-100218A), erastin-2 (HY-139087), iFSP1 (HY-136057), BTZ (HY-10227) and PEG300 (HY-Y0873) were purchased from MedChemExpress. ML-210 (S0788), MG-132 (S2619) and icFSP1 (E1535) were acquired from Selleck Chemicals. Rotenone (R8875), oligomycin (75351), antimycin A (A8674), L-BSO (B2515), N-acetyl cysteine (A9165), Na 2SeO 3 (S5261), CQ (C6628) and PEG400 (202398) were obtained from Sigma-Aldrich. FCCP (15218), MTT (21795), GSHee (14953), liproxstatin-1 (17730), IMP-1088 (25366), NSC 624206 (20569), FSEN1 (38025), viFSP1 (39927) and triacsin C (10007448) were obtained from Cayman Chemical Company."
A panel of murine B16-F0 and LN metastatic derivative cell lines were provided by the Reticker-Flynn Laboratory and are referenced by simplified names. B16F10 wild-type, Fsp1-KO, and Gpx4-KO cells were obtained from the Conrad Laboratory. Additional Fsp1-KO, Gclc-overexpression, Gclc-KO, and Nrf2-overexpression lines were generated. Human melanoma lines MeWo, SK-MEL-5, A375, murine YUMM3.3 and YUMM5.2, and HEK293T cells were purchased from ATCC. All cell lines were cultured in DMEM with 10% FBS and 1% penicillin-streptomycin, authenticated by STR profiling when applicable, and routinely tested for mycoplasma contamination. A broad set of chemicals and inhibitors was sourced from MedChemExpress, Selleck, Sigma-Aldrich, and Cayman Chemical Company.
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